摘要
镉 (Cd) 对植物造成严重损害。尽管已发现钙 (Ca) 信号对某些胁迫作出反应，但在 Cd 胁迫期间植物中 Ca 的定位和 Ca 信号的分子机制在很大程度上是未知的。在这项研究中，Ca 2+感应荧光报告基因 (GCaMP3) 转基因浮萍在 Cd 胁迫期间显示了Lemna turionifera 5511 (浮萍) 中的 Ca 2+信号响应。随后，通过透射电子显微镜研究了 Cd 胁迫期间Ca 2+的亚细胞定位，显示了 Ca 2+在液泡中的积累。此外，Ca 2+测量了 Cd 应力期间的流量。同时，外源谷氨酸（Glu）和γ-氨基丁酸（GABA）对浮萍的作用可以更好地阐明植物对Cd胁迫的信号运行机制。Cd胁迫下Ca 2+信号响应的分子机制表明，Cd处理促进植物细胞内Ca信号通道的正向响应，从而影响细胞内Ca含量。这些新的信号研究提供了在 Cd 胁迫期间重要的 Ca 2+信号分子机制。
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Mechanism of calcium signal response to cadmium stress in duckweed
ABSTRACT
Cadmium (Cd) causes serious damage to plants. Although calcium (Ca) signal has been found to respond to certain stress, the localization of Ca and molecular mechanisms underlying Ca signal in plants during Cd stress are largely unknown. In this study, Ca2+-sensing fluorescent reporter (GCaMP3) transgenic duckweed showed the Ca2+ signal response in Lemna turionifera 5511 (duckweed) during Cd stress. Subsequently, the subcellular localization of Ca2+ has been studied during Cd stress by transmission electron microscopy, showing the accumulation of Ca2+ in vacuoles. Also, Ca2+ flow during Cd stress has been measured. At the same time, the effects of exogenous glutamic acid (Glu) and γ-aminobutyric (GABA) on duckweed can better clarify the signal operation mechanism of plants to Cd stress. The molecular mechanism of Ca2+ signal responsed during Cd stress showed that Cd treatment promotes the positive response of Ca signaling channels in plant cells, and thus affects the intracellular Ca content. These novel signal studies provided an important Ca2+ signal molecular mechanism during Cd stress.